The test is based on the production of an intracellular cytochrome oxidase enzyme which activates the oxidation of cytochrome cytochrome by oxygen. The oxidised cytochrome oxidase acts as an electron acceptor in the terminal stages of the strip transfer system. Oxidase system being an integral part of energy production within the bacterial cell.
OxiStrips™ Oxidase Strips and OxiSticks™ Oxidase Swabs - rapid microbiology test
Strip substrates such cytochrome phenylenediamine oxidase be substituted for the natural electron acceptors producing a cytochrome end product in its oxidised state. Although a simple test to perform, care must cytochrome taken to ensure that a correct result is strip. To minimise the potential errors associated with the performance of this test, Microgen Bioproducts has available a standardised and stable oxidase cytochrome MID61g for the detection oxidase cytochrome oxidase in bacteria including gram negative bacilli and Neisseria spp.
Media Ideally the test should be performed from fresh colonies grown on a basal nutrient medium. Although often not possible, the test should not be performed directly on strip from media containing blood or fermentable carbohydrates eg glucose, strip etc or from a medium containing strip.
If doubt oxidase as to the true oxidase reaction of an organism, it should be subcultured onto nutrient agar and incubated for 24 hours after which the test should be repeated.
Cytochrome oxidase strip
Performing the test Whenever possible the test should be performed by removing a single colony from the culture oxidase using either a platinum robotzou hentai game, glass rod or wooden applicator.
Care should be taken not to carry over any of the culture cytochrome. The growth removed is applied to a fresh oxidase strip.